Analysis of local innate immune response to Eimeria acervulina using chicken intestinal cDNA microarray

Effect of vitamin A deficiency on host intestinal immune response to Eimeria acervulina in broiler chickens.

The effects of vitamin A (VitA) deficiency on the host intestinal immune response and disease susceptibility to coccidiosis were investigated in broiler chickens following oral infection with Eimeria acervulina (EA). Day-old male broilers were fed milo-soybean meal diets either with 8,000 IU VitA/kg feed (CONT) or without added VitA (A-DEF). At 25 d, a group of randomly selected birds from each...

Comparative Microarray Analysis of Intestinal Lymphocytes following Eimeria acervulina, E. maxima, or E. tenella Infection in the Chicken

Relative expression levels of immune- and non-immune-related mRNAs in chicken intestinal intraepithelial lymphocytes experimentally infected with Eimeria acervulina, E. maxima, or E. tenella were measured using a 10K cDNA microarray. Based on a cutoff of >2.0-fold differential expression compared with uninfected controls, relatively equal numbers of transcripts were altered by the three Eimeria...

Intestinal mucosal mast cell immune response and pathogenesis of two Eimeria acervulina isolates in broiler chickens.

Four experiments were conducted comparing intestinal immune responses to 2 isolates of Eimeria acervulina (EA), EA1 and EA2. In experiments 1 and 2, broiler chicks of 2 commercial breeds were divided into control (nonchallenged), EA1-, or EA2-challenged groups. On d 6 postchallenge (PC), changes in BW were determined, intestinal lesions were scored, and duodenal tissue was evaluated for morphom...

Analysis of chicken cytokine and chemokine gene expression following Eimeria acervulina and Eimeria tenella infections.

The expression levels of mRNA encoding a panel of 28 chicken cytokines and chemokines were quantified in intestinal lymphocytes following Eimeria acervulina and Eimeria tenella primary and secondary infections. Compared with uninfected controls, transcripts of the pro-inflammatory cytokines IFN-alpha, IL-1beta, IL-6, and IL-17 were increased up to 2020-fold following primary infection. By contr...

Inhibition of Eimeria acervulina Sporozoite Invasion by Rabbit and Chicken Antisera Using ISI Assay